Lanthanoid tags – from intrinsic susceptibility tensors to applications
The 1st colloquium of TRR-386 with Prof Daniel Häussinger from Basel on 12 January 2024 has been cancelled due to extensive obstructions in traffic. Prof Häussinger who is an expert in paramagnetic nuclear magnetic resonance spectroscopy on biomacromolecules in solution, will share his expertise im summer instead.
Lanthanoid chelating tags (LCTs) can be conjugated to biomacromolecules like proteins, nucleic acids or carbohydrates. Their strongly anisotropic paramagnetic susceptibility tensors lead to long range pseudocontact shifts (PCSs) in NMR spectra. We have designed and synthesized a variety of LCTs and exploit their properties to study the structures, dynamics and interactions of biomacromolecules in solution. The unique features of PCSs are their long range in the order of 100 Å, the precise three-dimensional information that is obtained and their applicability to large proteins exceeding 100 kDa, by using sensitive and straightforward HSQC type experiments.